Introduction of Tyramide Signal Amplification (TSA) to Pre-embedding Nanogold-Silver Staining at the Electron Microscopic Level

Novel application of tyramide signal amplification (TSA): ultrastructural visualization of double-labeled immunofluorescent axonal profiles.

Fluorescent immunocytochemistry (FICC) allows multiple labeling approaches when enzyme-based techniques are difficult to combine, such as in double-labeling experiments targeting small-caliber axonal segments. Nevertheless, the conversion of FICC to a product visible at the electron microscopic (EM) level requires labor-intensive procedures, thus justifying the development of more user-friendly...

Tyramide Signal Amplification Strategies for Fluorescence Labeling

Immunocytochemistry at the Electron-Microscopic Level

Staining enhancement and signal amplification

The method of tyramine amplification is based on the ability of HRP to catalyze the deposition of large amounts of biotinylated tyramine in the vicinity of the antigen-antibody reaction (i.e. the cellular site which contains the peroxidase detection complex). The method is based on earlier biochemical observations on the action of peroxidase upon tyramine and other related compounds (GROSS AJ a...

ترجمه و تحقیق کتاب an introduction to the history of mysticism

پایان نامه ی ارائه شده ترجمه ی کتاب «an introduction to the history of mysticism» نوشته ی مارگارت اسمیت که وی پژوهشگر، خاورشناس و محقق برجسته ی تصوف و عرفان می باشد. موضوع اساسی و مهم این کتاب عرفان در ادیان مختلف است. نویسنده در ابتدا این کلمه را ریشه یابی کرده و سپس تعریفی از آن ارائه داده و آن را در ادیان مختلف مورد بحث قرار داده است و در هر فصل کتاب به معرفی عرفای آن دین یا عصر خاصی پرداخته...